Two precise, simple, accurate, reproducible, rapid and economic UV Spectrophotometric methods have been developed for simultaneous estimation of amlodipine and losartan in tablet dosage form by using distilled water as a solvent. Method I was based on formation and solving of simultaneous equation, known as Vierodt’s method. Losartan potassium and amlodipine besylate shows absorbance maximas at 250 nm and 240 nm respectively, so absorbance was measured at the same wavelength for the estimation of losartan potassium and amlodipine besylate in tablet combination. Method II was based on principle of Q-analysis, known as absorbance ratio method. Absorbance was measured at 254 nm (Isobestic point) and 250 nm (λmax of losartan). Losartan potassium and amlodipine besylate obeys Beer’s law in the concentration range of 0 to 30 µg/ml. Methods are validated according to ICH guidelines and can be applied for routine analysis of drugs in tablet dosage form.
Loading....